Peformance Comparison of Imaging Plate Detector Systems for Macromolecular Crystallography

Performance Comparison of Imaging Plate Detector Systems for Macromolecular Crystallography

The Woodlands, TX — March 19, 2004. Rigaku today announced the publication of a new Application Note that compares and contrasts the performance of new high-throughput imaging plate (IP) technology to the established standard in IP detectors. The publication also details how the use of a 3-plate detector system provides throughput, for macromolecular structure determinations, that is similar to CCD detectors while maintaining the desirable characteristics of a large aperture with extended linear dynamic range.

Titled "Imaging plate detector performance comparison: R-AXIS HTC vs. R-AXIS IV++," the two page document describes the result of an experiment in which the performance of Rigaku's new 3-plate (R-AXIS HTC) imaging plate detector is compared to the established standard R-AXIS IV++ detector for data collection on thaumatin and cholesterol oxidase, two macromolecular samples with different levels of difficulty. For the more difficult cholesterol oxidase case, a nearly 28% reduction in data collection time was achieved without sacrificing data quality. Data processing results are given to substantiate the claims.

Rapid data collection has become a high priority for protein crystallography in high throughput environments. Rigaku's R-AXIS HTC, with its novel 3-plate design for concurrent expose, read, and erase operations, overcomes this limitation. While one plate is exposed to the X-ray diffraction pattern, the exposed image on a second plate is read out, and, at the same time, a third plate is positioned in the erase station and is being prepared for the next exposure.

An imaging plate is a film-like radiation image sensor comprised of specifically designed phosphor that trap and store X-ray radiation energy. Energy from X-ray photons impinging on such an IP are stored and stable until scanned with a laser beam during the read operation. A flexible stainless steel belt is used as both a support and transport system for the imaging plates in the R-AXIS detectors. IP detectors are the established standard, for home laboratory settings, within the field of macromolecular crystallography.

Rigaku—Leading With Innovation

Since its inception in Japan in 1951, Rigaku has been at the forefront of analytical and industrial instrumentation technology. Rigaku and its subsidiaries form a global group focused on life sciences and general purpose analytical instrumentation. With hundreds of major innovations to their credit, Rigaku companies are world leaders in the fields of small molecule and protein crystallography, X-ray spectrometry and diffraction, X-ray optics, as well as semiconductor metrology. Rigaku employs over 1,100 people in the manufacture and support of its analytical equipment. Its products are in use in more than 70 countries—supporting research, development, and quality assurance activities. Throughout the world, Rigaku continuously promotes partnerships, dialog, and innovation within the global scientific and industrial community.

For further information, contact:

Joseph D. Ferrara, Ph.D.
VP Product Marketing and CSO
Rigaku Americas Corporation
Tel: (281) 362-2300
eMail: Joseph D. Ferrara