Automated in situ crystallography

XtalCheck

The XtalCheck system is an automated tool for performing in situ crystallography experiments on your existing X-ray diffraction system. Protein crystallography often requires screening large numbers of crystals to identify samples that are suitable for X-ray diffraction experiments. Specifically, crystallographers usually loop and cryo-freeze samples for X-ray screening to identify whether the sample contains a protein or salt and to evaluate diffraction resolution, mosaicity and other crystal parameters. This iterative process of mounting and screening of many samples is time consuming and rarely automated. The XtalCheck system addresses this bottleneck by automating diffraction data collection for crystals directly from SBS format crystallization plates.

The XtalCheck system includes software that facilitates both visual and diffraction imaging of crystallization experiments. With the XtalCheck system, one can easily survey many crystallization experiments by eliminating the need to harvest and cryo-cool samples. Moreover, one can perform serial crystallography experiments, by collecting data from multiple crystals, to achieve complete data sets that can be used for structure solution.

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Features

  • Quickly survey crystals prior to cryo-freezing
  • Simple queuing of crystal objects for diffraction screening
  • Data collection for sample queue progresses without user intervention
  • Assign multiple crystal objects per drop
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