
HyPix-3000 is a next-generation two-dimensional semiconductor hybrid pixel array detector (HPAD), pixel size of 100 um², designed specifically to meet the needs of the home lab diffractionist.
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Protein crystallography, small molecule crystallography, SAXS, micro-diffraction, phase contrast imaging
The MicroMax™ 007 HF is the most widely used home lab X-ray source for protein crystallography and a popular source for small molecule crystallographers who need the additional flux of a rotating anode generator. The original MicroMax™ 007 introduced the world to the concept of a sub-100 micron focal spot size along the increased brilliance that can be more effectively used to illuminate small crystals. The current HF version increases the power loading by 50% over the original model providing an X-ray source that rivals second generation synchrotron sources - but with better beam stability and, of course, easy access.
“We recently replaced our optics-equipped 5 kW rotating anode with a Rigaku Micromax-007 HF and VariMax HF optics. We continue to use our original IP detector. Having been involved in the purchase of several generation of generator I'm used to the assurances of ‘This is almost as powerful as a synchrotron beam line.' This time is unusual in that the promise has been fulfilled. We have seen exposure times go from 25-30 minutes to 30 seconds, and visible diffraction from crystals we never previously expected to diffract at home. The new generator has made a major difference in our ability to investigate macromolecular crystallographic problems.” — Dr Paul Taylor, University of Edinburgh, Scotland
X-ray generator
Anode assembly
Tube tower assembly
X-ray source data
A special version of the 007 (MicroMax 007 HF DW) is available with a dual banded anode. The target of choice is selected through an anode translation adjustment and this additional mechanism limits the generator to one X-ray port. The user can switch between wavelengths without realigning the system when used in conjunction with a VariMax DW optic. The anode of the MicroMax-007 HF DW is double banded with Cu/Cr or Cu/Mo target combinations available as standard configurations and on special request, Kα and Kβ lines of Cr, Cu, Mo, and Ag are available in custom combinations.
Various optics are available for the MicroMax 007 HF depending on the desired beam characteristics and wavelength of the anode target.
The VariMax optics product line is the most suitable for single crystal applications. These optical systems all feature a patented, continuously adjustable divergence slit assembly that enables users to adjust the X-ray beam divergence to allow for optimal spot separation for each crystal. The LFE designation refers to optics that are manufactured with a special low figure error substrate, resulting in a more efficient optic with a smaller beam size.
Application | |||
---|---|---|---|
VariMax HF | Protein crystallography with Cu radiation and small crystals | ||
VariMax HF LFE | Protein crystallography with Cu radiation and small crystals | ||
VariMax VHF | Protein crystallography with Cu radiation and very small crystals | ||
VariMax HF LFE | Protein crystallography with Cu radiation and very small crystals | ||
VariMax Cr | Protein crystallography with Cr radiation for SAD phasing | ||
VariMax Ag | Small molecule crystallography with Ag radiation for high Z electron density studies. Available in Kα and Kβ | ||
VariMax Co | Protein crystallography with Co radiation for SAD phasing | ||
VariMax Mo | Small molecule crystallography with Mo radiation and small crystals |
Radiation enclosures can be designed and built to meet your specifications. Whether you want a walk-in enclosure that gives you maximum maneuverability when working around the X-ray instrumentation or a table top enclosure to give maximum safety protection when the X-ray equipment is housed in a working laboratory, Rigaku can provide what you need.
The MicroMax 007 HF is provided with a cabinet that contains the electronics and a breadboard top to position the anode head. Depending on your needs, extension tables can be added to either side of the electronics cabinet to give you flexibility in utilizing the left port, the right port or both ports. Extension tables have a optical bench breadboard top for maximum stability of the X-ray beam.
The MicroMax 007 HF can be used for a variety of applications where a small stable X-ray beam is desired. Final beam characteristics are defined by the optic that is used. Many different models of optics are available based on whether the desired beam is focused or parallel, and if focused, what is the desired beam size at the focal point and divergence of the beam.
- Using the home source to good advantage by Anna Plechanovona (University of Dundee) and Jim Naismith (University of St Andrews)
- Dr. Arwen Pearson from University of Leeds discusses her new HighFlux HomeLab system
- The decision to purchase from Rigaku by Professor Bill Hunter, College of Life Sciences, University of Dundee
Excerpt: The new cell was a = 50.5 Å b = 65.1 Å c = 189.6 Å with space group P2₁2₁2₁. Old hands with burnt fingers will recognize that this looks very bad. The 90° image will be overlapped to an unusable extent as the long axis is parallel to the beam.The in-‐house data collection set up came to the rescue. First we drove the χ axis (we have a partial χ goniometer) to its maximum deflection (45°) to try to move the long axis to a more amenable orientation. The image looked more hopeful but we knew we would still have to sacrifice resolution to ensure processability ( a painful compromise).
We were saved by another feature of our home source, the swing out angle. It allows higher resolution data to be collected with the detector positioned further back allowing better separation of spots. Such orientations of the detector are handled automatically in processing. By pushing the detector out 20° we obtained a new image. Although overlapping a little, the data were easily processed, we collected the data in three passes (different settings on the goniostat) to ensure completeness and redundancy. The new data gave spectacular quality maps and allowed us to tell the story.
The decision to purchase from Rigaku was based on several criteria. Our group in Dundee requires equipment that would provide excellent in-house data collection including suitable automation, where high throughput is needed in support of early stage drug discovery. We need the capability to obtain highly redundant data for in-house anomalous dispersion phasing and the capacity to test and characterise large numbers of crystals from our more challenging problems in a manner that ensures we can optimise use of synchrotron time. The configuration from Rigaku matched perfectly to our multi-purpose requirements. My very positive experience of support from the company for almost 20 years now, also provided a degree of security in the decision.