Protein Crystallography
 
 
Protein Crystallography
 

We are thrilled with the Rigaku X-ray generator and Saturn CCD detector combination in our X-ray Core Facility at the University of Puerto Rico.

Recently, we were able to collect 1.09 Å data on our Rigaku system that allowed us to see the conformational changes that take place in blackfin tuna myoglobin upon S-nitrosylation (Journal of Biological Chemistry, 282 (2007) 19773).

This post-translational protein modification is extremely sensitive to X-ray radiation, so we were not able to collect synchrotron data and had to use our home source. The sensitivity of the Saturn CCD detector allowed us to minimize the exposure times and still collect atomic resolution data that was necessary to deconvolute multiple conformations of the S-nitrosylated cysteine.

—Dr. Eric R. Schreiter
Director, X-ray Crystallography Core Facility
Protein Research and Development Center
University of Puerto Rico

   

Macromolecular Crystallography Webinar training series

Welcome to the Rigaku Life Sciences Webinar Series. This free webinar series is designed to provide educational and training materials in X-ray crystallography methods. Each webinar will address a new topic ranging from optimization of crystallization conditions to solving the phase problem. We invite anyone to submit topic suggestions for future webinars and hope that you'll provide feedback for previous webinars. 

Most recent webinar:

  • Date: Thursday, January 21, 2010 
  • Time: 10:00 AM - 11:00 AM EST (3:00 PM - 4:00 PM GMT)
  • Title: Processing X-ray diffraction data with HKL - an in depth exploration
  • Presenters: Wladek Minor


Wladek Minor

In this webinar, Wladek Minor will explore the depths of HKL-2000 and the new HKL-3000 software packages. Designed for X-ray diffraction image processing, HKL2000 is used by more than 1200 labs worldwide. The paper: "Processing of X-ray diffraction data collected in oscillation mode" Methods in Enzymology (1997) 275: 307-326 that describes the program has been cited more than 19,500 times throughout the scientific literature. HKL and HKL-2000 components were written by Zbyszek Otwinowski (Professor, UT Southwestern in Dallas) and Wladek Minor (Professor, University of Virginia). Please join us to learn more about getting the most from this X-ray diffraction image processing package.

Download the slides from this presentation

To register for the webinar, please fill out this form.

 

Past webinars:

To view and/or download past webinars click here

To find information about future webinars, check this web site frequently. Upcoming webinars are also included in our monthly Life Sciences newsletter. If you would like to receive this monthly newsletter send email to info@rigaku.com.

Other training courses:

Rigaku Americas is pleased to offer valuable training sessions to teach you and your staff how to keep your Rigaku diffraction equipment running in excellent shape and how to extend the lifetime of your equipment by hands-on training from service specialists. Learn more.