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The development and evaluation of a new SAXS system for biological samples

AppNote BX001 Biological SAXS: Development and evaluation of a Kratky system

Background

SAXS (Small Angle X-ray Scattering) is a well-known method for studying the size and the shape of particles in solution. More recently, SAXS has seen a resurgence as a useful tool to study biological samples. X-ray crystallography is a fundamental technique for obtaining atomic resolution structural information for macromolecules. However, the requirement for diffraction quality crystals often limits its efficacy for studying protein complexes or systems with inherent structural disorder. In cases where crystallographic methods have failed, SAXS provides a complementary tool for extracting structural information from biological systems. In particular, SAXS proves ideal for studying partially disordered macromolecules, for monitoring structural changes in response to environmental perturbations, and for monitoring conformational changes due to ligand binding.

SAXS products from Rigaku

A customized single crystal X-ray diffraction system that has the capabilities of utilizing both ports of a rotating anode X-ray source.

Small and wide angle X-ray scattering instrument designed for nano-structure analyses

Small and wide angle X-ray scattering instrument designed for nano-structure analyses

A modernized 2D Kratky system that eliminates data corrections required of traditional systems

Small angle X-ray scattering (SAXS) Kratky camera system

Special CMF optic designed for SAXS instrumentation

Multipurpose products from Rigaku

Advanced state-of-the-art high-resolution XRD system powered by Guidance expert system software

Highly versatile multipurpose X-ray diffractometer with built-in intelligent guidance

High-performance, multi-purpose XRD system for applications ranging from R&D to quality control