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BioSAXS-1000: A new tool for gaining structural insight when crystallographic methods are not enough

AppNote BX002: BioSAXS vs protein crystallography

Background

X-ray crystallography is a fundamental technique for obtaining atomic resolution structural information about macromolecules. However, the requirement for diffraction quality crystals often limits its efficacy for studying protein complexes or systems with inherent structural disorder. In cases where crystallographic methods fall short, small angle X-ray scattering (SAXS) provides a complementary tool for extracting structural information from biological systems. In particular, SAXS proves ideal for studying partially disordered macromolecules, for monitoring structural changes in response to environmental perturbations, and for monitoring conformational changes due to ligand binding. It also provides a useful tool for probing molecular interactions, such as those associated with crystallization. Here we introduce the first commercial system specifically designed for high-throughput solution scattering experiments with macromolecules, the BioSAXS-1000. Comprised of specially designed focusing optics and a Kratky block, the BioSAXS-1000 system eliminates smearing issues common to traditional Kratky cameras. The system includes a high sensitivity pixel array detector and intuitive data collection software that includes full automation of the Kratky alignment hardware. Together, the many features of the BioSAXS-1000 system allow for synchrotron-quality SAXS data from a home laboratory source in as little as 15 to 30 minutes for most protein samples.

SAXS products from Rigaku

Small angle X-ray scattering (SAXS) Kratky camera system

Small and wide angle X-ray scattering instrument designed for nano-structure analyses

Small and wide angle X-ray scattering instrument designed for nano-structure analyses

A modernized 2D Kratky system that eliminates data corrections required of traditional systems

Special CMF optic designed for SAXS instrumentation

Multipurpose products from Rigaku

Single crystal X-ray diffractometer with custom enclosure and flexibility for easy integration of accessory components

Advanced state-of-the-art high-resolution XRD system powered by Guidance expert system software

Highly versatile multipurpose X-ray diffractometer with built-in intelligent guidance

High-performance, multi-purpose XRD system for applications ranging from R&D to quality control