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Study of microcrystalline protein suspensions using the BioSAXS-2000

AppNote BX007: Using the BioSAXS to study microcrystalline protein suspensions

Background

X-ray scattering and diffraction techniques are useful analytical tools when investigating protein in liquid form, either as so lution samples or as crystalline suspensions. For example, small angle X-ray scattering can be used to understand how proteins behave in solution and for calculation of structural parameters and models. Data from microcrystalline suspensions can be used to evaluate sample properties prior to measurement at an xFEL. X-ray data from microcrystalline suspensions can also guide the optimization of biological pharmaceuticals to combat human disease. For example, the literature contains many studies of insulin and immunoglobulins with the aim of optimizing dr ug delivery systems. In such cases, the ideal product is one that delivers a high concentration of a pharmaceutical product, albeit in low volumes. In this study, we characterize macromolecular suspensions using the BioSAXS-2000 system.

SAXS products from Rigaku

Small angle X-ray scattering (SAXS) Kratky camera system

Small and wide angle X-ray scattering instrument designed for nano-structure analyses

Small and wide angle X-ray scattering instrument designed for nano-structure analyses

A modernized 2D Kratky system that eliminates data corrections required of traditional systems

Special CMF optic designed for SAXS instrumentation

Multipurpose products from Rigaku

Single crystal X-ray diffractometer with custom enclosure and flexibility for easy integration of accessory components

Advanced state-of-the-art high-resolution XRD system powered by Guidance expert system software

Highly versatile multipurpose X-ray diffractometer with built-in intelligent guidance

High-performance, multi-purpose XRD system for applications ranging from R&D to quality control