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In Situ S-SAD data collection and phasing of native lysozyme crystals using the PlateMate

AppNote PX013 In situ single crystal X-ray diffraction study of lysozyme using the PlateMate

Background

The PlateMate is a simple, inexpensive tool for rapid screening of crystals within crystallization plates. The PlateMate is equipped with a standard IUCr fitting for easy installation on most Rigaku Oxford Diffraction goniometers, and supports SBS format crystallization plates. With the PlateMate, you can easily answer the questions: “Is it a protein crystal or a salt crystal?” and “How far does my crystal diffract at room temperature, prior to cryofreezing?” In addition, the PlateMate enables you to collect complete, or nearly complete, data sets for many protein crystals without ever removing the crystal from the crystallization drop. The PlateMate also provides an important time gain for fragment screening projects and for screening crystals for bound compounds in protein active sites. Herein, we describe a data collection example using the PlateMate.

Protein crystallography products from Rigaku

A customized single crystal X-ray diffraction system that has the capabilities of utilizing both ports of a rotating anode X-ray source.

A microfocus sealed tube single crystal X-ray diffractometer that is low maintenance and perfect for screening and evaluating protein crystals.

A single crystal X-ray diffractometer with high-flux microfocus rotating anode X-ray generator and direct detection, HPC X-ray detector.

A versatile and high-flux dual-wavelength (DW) X-ray diffractometer with an HPC X-ray detector for multipurpose diffraction experiments

Single wavelength Confocal Max-Flux (CMF) optics for single crystal diffraction

Automated tool for performing in situ crystallography experiments on existing X-ray diffractometers

Small angle X-ray scattering (SAXS) Kratky camera system

User-inspired data collection and data processing software for small molecule and protein crystallography

A unique curved single crystal X-ray diffraction detector based on direct X-ray detection technology with the highest 2θ range at a single position available for the home lab.

Extremely low noise, air-cooled X-ray detector based on direct X-ray detection technology.

High-resolution X-ray detectors for X-ray diffractometers from DECTRIS

Automated crystal transport, orientation and retrieval robot

The smallest detachable motorized goniometer head on the market