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In situ screening and data collection using the XtalCheck system

AppNote PX017 In situ single crystal X-ray diffraction studies using the XtalCheck

Background

Protein crystallography often requires screening large numbers of crystals to identify samples that are suitable for X-ray diffraction experiments. Specifically, crystallographers usually loop and cryofreeze samples for X-ray screening to identify whether the sample contains a protein or salt and to evaluate diffraction resolution, mosaicity and other crystal parameters. This iterative process of mounting and screening of many samples is time consuming and rarely automated.

The XtalCheck system addresses this bottleneck by automating diffraction data collection for crystals directly from SBS format crystallization plates. With the XtalCheck system, one can quickly and easily survey many crystallization experiments by eliminating the need to harvest and cryo-protect samples. Moreover, one can perform serial crystallography experiments, by collecting data from multiple crystals, to achieve complete data sets that can be used for structure solution. The system also includes automated imaging of crystallization drops with a queuing system that was developed to use in laboratories with an existing X-ray diffraction system.

Protein crystallography products from Rigaku

Fast, flexible single crystal X-ray diffractometer with the latest generation sources and HPC X-ray detectors, perfect for any crystallography lab

Single crystal X-ray diffractometer with high-flux microfocus rotating anode X-ray generator

Versatile and high-flux dual-wavelength (DW) X-ray diffractometer with HPC X-ray detector for multipurpose diffraction experiments

Single crystal X-ray diffractometer with custom enclosure and flexibility for easy integration of accessory components

Small angle X-ray scattering (SAXS) Kratky camera system

Automated crystal transport, orientation and retrieval robot

User-inspired data collection and data processing software for small molecule and protein crystallography

Single wavelength Confocal Max-Flux (CMF) optics for single crystal diffraction

Automated tool for performing in situ crystallography experiments on existing X-ray diffractometers

Low noise, sensitive, air-cooled X-ray detector for superior X-ray diffraction data quality

High resolution X-ray detectors for X-ray diffractometers from DECTRIS