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In-situ Data Collection and Structure Solution Using the XtalCheck-S System

AppNote PX020 In-situ single crystal X-ray diffraction studies using the XtalCheck-S

Background

Protein crystallography often requires screening large numbers of crystals to identify samples that are suitable for X-ray diffraction experiments. Specifically, crystallographers usually loop and cryofreeze samples for X-ray screening to identify whether the sample contains a protein or salt and to evaluate diffraction resolution, mosaicity and other crystal parameters. This iterative process of mounting and screening many samples is time consuming and rarely automated. 

The XtalCheck-S system addresses this bottleneck by automating diffraction data collection for crystals directly from SBS format crystallization plates. With the XtalCheck-S system, one eliminates the need to harvest and cryo-protect samples and, thus, can quickly and easily survey many crystallization experiments. In-plate screening has the added advantage that it reduces the risk of damaging fragile or sensitive crystals by eliminating the need to remove them from mother liquor for X-ray data collection. Moreover, one can perform complete crystallography experiments by collecting data from multiple crystals in random orientations to achieve a complete set of reflection that can be used for structure solution.

Protein crystallography products from Rigaku

Fast, flexible single crystal X-ray diffractometer with the latest generation sources and HPC X-ray detectors, perfect for any crystallography lab

Single crystal X-ray diffractometer with high-flux microfocus rotating anode X-ray generator

Versatile and high-flux dual-wavelength (DW) X-ray diffractometer with HPC X-ray detector for multipurpose diffraction experiments

Single crystal X-ray diffractometer with custom enclosure and flexibility for easy integration of accessory components

Small angle X-ray scattering (SAXS) Kratky camera system

Automated crystal transport, orientation and retrieval robot

User-inspired data collection and data processing software for small molecule and protein crystallography

Single wavelength Confocal Max-Flux (CMF) optics for single crystal diffraction

Automated tool for performing in situ crystallography experiments on existing X-ray diffractometers

Low noise, sensitive, air-cooled X-ray detector for superior X-ray diffraction data quality

A unique curved single crystal X-ray diffraction detector

High resolution X-ray detectors for X-ray diffractometers from DECTRIS