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Resolving Reflections from Large Unit Cells at Short Detector Distances

AppNote PX025: Resolving Reflections from Large Unit Cells at Short Detector Distances

Introduction

Home lab X-ray systems use confocal multilayer optics to produce a small X-ray beam, but often at the expense of higher beam divergence (up to 10 milliradians) that can typically cause reflection overlap when collecting on protein crystals with large unit cells. The Rigaku Oxford Diffraction XtaLAB Synergy systems address this issue by combining two new technologies: the HyPix-6000HE hybrid photon counting (HPC) detector, featuring a small pixel size (100 μm x 100 μm) and a single pixel point-spread function, and a slit mechanism that reduces the beam divergence on demand. Here, we show how reflections along the longest unit cell axis of catalase (~230 Å) can be resolved by using the slit at a short detector distance of 70 mm. Furthermore, we show that a high quality data set to 1.9 Å can be collected overnight and produce an excellent molecular replacement solution.

Protein crystallography products from Rigaku

A customized single crystal X-ray diffraction system that has the capabilities of utilizing both ports of a rotating anode X-ray source.

A microfocus sealed tube single crystal X-ray diffractometer that is low maintenance and perfect for screening and evaluating protein crystals.

A single crystal X-ray diffractometer with high-flux microfocus rotating anode X-ray generator and direct detection, HPC X-ray detector.

A versatile and high-flux dual-wavelength (DW) X-ray diffractometer with an HPC X-ray detector for multipurpose diffraction experiments

A leading provider of reagent kits, stock solutions, proteins, and accessories for protein researchers.

Small angle X-ray scattering (SAXS) Kratky camera system

User-inspired data collection and data processing software for small molecule and protein crystallography

Automated tool for performing in situ crystallography experiments on existing X-ray diffractometers

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A unique curved single crystal X-ray diffraction detector based on direct X-ray detection technology with the highest 2θ range at a single position available for the home lab.

Extremely low noise, air-cooled X-ray detector based on direct X-ray detection technology.

High-resolution X-ray detectors for X-ray diffractometers from DECTRIS

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The smallest detachable motorized goniometer head on the market