Skip to main content

Sulfur SAD phasing of thaumatin in 10 minutes

AppNote PX026: Sulfur SAD phasing of thaumatin in 10 minutes with the HyPix-Arc 150° and XtaLAB Synergy-DW


T. danielli Thaumatin, a sweet-tasting protein often used as a sweetner, features an axis of ~150 Å. It is well characterised by protein crystallography and makes a good candidate for testing new equipment as it becomes available. Recently Rigaku Oxford Diffraction introduced a new detector - the HyPix-Arc 150°. The new detector provides extremely high theta coverage which can be used for fast collection of Friedel pairs. The detector features HPC technology for the highest accuracy of intensity measurement for both weak and strong signal ensuring that anomalous signal is well measured for S-SAD phasing applications. This note demonstrates fast S-SAD phasing of T. danielli Thaumatin.

Protein crystallography products from Rigaku

A customized single crystal X-ray diffraction system that has the capabilities of utilizing both ports of a rotating anode X-ray source.

A microfocus sealed tube single crystal X-ray diffractometer that is low maintenance and perfect for screening and evaluating protein crystals.

A single crystal X-ray diffractometer with high-flux microfocus rotating anode X-ray generator and direct detection, HPC X-ray detector.

A versatile and high-flux dual-wavelength (DW) X-ray diffractometer with an HPC X-ray detector for multipurpose diffraction experiments

A leading provider of reagent kits, stock solutions, proteins, and accessories for protein researchers.

Small angle X-ray scattering (SAXS) Kratky camera system

User-inspired data collection and data processing software for small molecule and protein crystallography

Automated tool for performing in situ crystallography experiments on existing X-ray diffractometers

Robotic sample changer to provide unattended data acquisition, enhanced productivity and standardized workflow to your research environment.

A unique curved single crystal X-ray diffraction detector based on direct X-ray detection technology with the highest 2θ range at a single position available for the home lab.

Extremely low noise, air-cooled X-ray detector based on direct X-ray detection technology.

High-resolution X-ray detectors for X-ray diffractometers from DECTRIS

Automated crystal transport, orientation and retrieval robot

The smallest detachable motorized goniometer head on the market