The quality of cell culture media used for the growth of cells under controlled conditions plays an important role in the efficiency of biopharmaceutical production. Cell culture conditions vary widely, including form factors – typically liquid or powder form – and in molecular makeup. These samples are most often complex mixtures of amino acids, sugars, salts, and vitamins, with some components in very high concentration while others are extremely low and variant. Analytical techniques for cell media also vary, as differentiating media types can be challenging. The biopharmaceutical industry strives for more understanding of the effect of media on cell health and batch success, and to limit the negative contribution of variance that may be sourced in their purchased cell culture media materials.
A selection of common media powders were scanned with a handheld Rigaku Progeny 1064 nm Raman analyzer and further studies were done to determine selectivity of a potential library.
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Handheld Raman for raw material identification and finished product authentication using 1064 nm Raman analysis.