Small and wide angle X-ray scattering Kratky system

X-ray scattering of macromolecules


Rigaku's BioSAXS-2000nano SAXS camera is designed specifically to meet the needs of the structural biologist. Based on a patented two-dimensional Kratky design, the BioSAXS-2000 takes up much less space than a conventional 3-pinhole camera but offers better flux characteristics. Best of all, the BioSAXS-2000nano can be mounted on the open port of a Rigaku rotating anode X-ray generator, taking full advantage of existing infrastructure, or it can be mated to a Rigaku microfocus sealed tube X-ray source. The BioSAXS AUTO configuration incorporates two time-saving features: an Automatic Sample Changer for unattended overnight operation and an Automatic Analysis Pipeline based on the world standard ATSAS package from EMBL Hamburg.

No need to wait for beamtime at a synchrotron

The BioSAXS-2000nano is equipped with the new OptiSAXS optic, a doubly focusing multilayer optic whose focus is at the detector. The OptiSAXS provides better than two fold improvement in flux compared to its predecessor resulting in higher brilliance at the sample position and data collection times in the range of minutes. Thus, the BioSAXS-2000nano brings a beamline experience to home laboratory SAXS experiments.

Optional accessories

  • Automatic Sample Changer (ASC) with support for up to 96 samples. See Accessories tab for more details.
  • Automatic Analysis Pipeline (AAP) based on ATSAS, the world’s most popular SAXS analysis software developed at EMBL Hamburg. See Accessories tab for more details.
  • Additional stages for SAXS measurement for materials samples.


  • Easily installed on the open port of an existing generator or can be installed with your choice of X-ray source: Microfocus sealed tube, MicroMax-007 HF, FR-X
  • Point focusing optics eliminates smearing issues common to traditional Kratky cameras.
  • All system components are motorized for control from control computer
  • Photodiode beamstop for intensity measurements and sample absorption correction
  • Sample temperature control included with system
  • 2D Kratky collimation allows one to achieve low q measurements with no realignment
  • SAXSLab data collection and processing software

Below are the specifications for the BioSAXSnano protein small angle scattering X-ray system from Rigaku.

  • Incident Beam: OptiSAXS CMF focusing optic
  • Collimation: 2D Kratky (X stage and tilt, motorized)
  • Beam size at Sample: Variable Width x 1.5 mm²
  • Sample Stage: 3 capillaries (X, Y stage, motorized) with a flow cell option
  • Sample Volume: 20 – 30 μL
  • Sample Temperature Control
  • Beam Stop: PIN diode detector (motorized)
  • q-range: 0.006 Å⁻¹ - 0.6 Å⁻¹
  • Detector: Rigaku HyPix-3000 Hybrid Pixel Array Detector (pixel size: 100 μm, active area: 77.5 mm X 30.5 mm)

Because data collection is faster with the BioSAXS-2000, automated tools for sample mounting, data collection and data analysis become especially important. The BioSAXS AUTO package accomplishes each of those tasks with the Automated Sample Changer (ASC) for automatic sample loading and data collection, and the Automated Analysis Pipeline (AAP) for automatic data processing and analysis.

EZ Aligner

The EZ Aligner tool allows for safe and remote alignment for VariMax or OptiSAXS optics. As the name implies, the EZ Aligner makes optimization of beam intensities an easy task. Simply set up your pin diode and pinhole, then use the EZ Aligner remote to optimize the beam from a safe place outside the radiation enclosure. Each EZ Aligner remote produces a radio signal strong enough to penetrate enclosure material or from another room. The EZ Aligner kit includes a remote control that communicates to 4 motors attached to optic micrometers to adjust optic rotation and translation.

EZ Aligner
BioSAXS Automatic Sample Handling

The ASC provides unattended sample mounting and data collection for those labs who want to take advantage of the higher throughput capabilities offered by the BioSAXS-2000nano. The ASC supports samples supplied in 96-well plates or 0.2 mL PCR tube arrays and seamlessly integrates with the hardware and SAXSLab software control package. Solution samples can be foil-sealed to ensure that your samples won’t evaporate prior to data collection. The ASC also includes support for temperature controlled sample storage and for multiple cleaning solutions and cleaning protocols. New features include a data collection wizard for quick and easy setup for 96 samples, configurable custom washes and dual syringe speeds for reduced dead-time between samples, and an option to incorporate turbo pumps which pump three times faster than the standard pumps. In all, the feature set of the ASC offers the benefit of quick preparation for automated experiments and optimized parameters to increase sample throughput.

BioSAXS Automatic Sample Handling
Automatic Data Analysis Pipeline

The AAP uses automated programs in the ATSAS 2.7 version to provide the following automatic analyses for each sample:

  • Automatic sample evaluation and aggregation identification
  • Automatic data averaging and analysis
  • Automatic Guinier plot generation and calculation of Rg and I(0)
  • Automatic Kratky plot generation
  • Automatic Porod volume and molecular weight (MW) calculation
  • Automatic P(r) calculation with report of real space Rg and Dmax
  • Automatic estimation of qmax from useful Shannon channels
  • Automatic MW calculation by the Volume of Correlation, Vc
  • Automatic envelope calculation, averaging and analysis
  • Automatic shape classification
  • PDF report generator and easy review of previous AAP runs

The AAP thus allows for quick evaluation of sample quality, with color-coded results so that you can easily identify well-behaving samples versus those that exhibit aggregation and concentration effects.
Automatic Data Analysis Pipeline


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A video of the BioSAXS-2000, the protein small angle X-ray scattering system from Rigaku

If you are unable to view this video, click here to download it (262 MB).


Focuses and monochromatizes an X-ray beam for small angle X-ray scattering

Rigaku's OptiSAXS optic is the latest generation of Cofocal Max-Flux® (CMF) optic for the BioSAXS-2000nano SAXS system configured with a Rigaku microfocus rotating anode generator. The long length of the OptiSAXS optic allows it to capture more of the X-ray beam from the microfocus generator resulting in higher flux. As a result, the OptiSAXS optic delivers an intense, monochromatic X-ray beam with greater than two-fold higher flux compared to its predecessor.


  • Graded, multilayer confocal optic with increased length for maximum beam intensity.
  • Double-bounce beam geometry for optimum beam purity
  • Designed to achieve maximum performance when coupled with Rigaku microfocus X-ray generators

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