Measuring damage to the corneal layer of skin

Human surface skin consists of basal layer, prickle layer, granular layer and corneal layer, and it repeats metabolism in a period of 28 days (Fig. 1). The corneal layer on the surface of the skin plays important roles such as a barrier function to protect the skin from outside damage and a moisturizing function. To reveal the structure in the corneal layer such as the lamellar structure is indispensable for studying the effectiveness of a cosmetic product and a pharmaceutical product. The spacing of the periodic lamellar structure is longer than the lattice spacing of the normal crystalline system, so the diffraction lines appear in the small angle region.

NANO-Viewer can measure the small angle region at high sensitivity, and it is an X-ray structure evaluation instrument that can evaluate the structure in the molecular level.

Human surface skin consists of basal layer, prickle layer, granular layer and corneal layer

Figure 1: Structure of the skin surface

When the corneal layer is healthy, the diffraction lines of the lamellar structure are confirmed up to 5th orders (Fig. 2). However, if the corneal layer is exposed to a large amount of ultraviolet light or other hazardous environment, it loses the barrier function and moisturizing ability, and suffers damage. As a result, the structure of the corneal layer is destroyed and the spacing of the lamellar structure varies widely; so clear diffraction lines are not detected after if the corneal layer suffers damage.

Change of the profile of the corneal layer due to damage
Figure 2: Change of the profile of the corneal layer due to damage



The S-MAX3000 pinhole SAXS camera design is available with a choice of conventional or high brilliance X-ray sources. A 3-meter, fully evacuated camera length provides both high intensity and high resolution. Coupled with a fully integrated 2-dimensional multi-wire proportional counter, the system is capable of making highly sensitive measurements from both isotropic and anisotropic materials. A wide range of sample stage attachments provide maximum flexibility in controlling environmental sample conditions during measurement. Read more about S-MAX3000...