HighFlux HomeLab 用户感言

Using the home source to good advantage

by Anna Plechanovona (University of Dundee) and Jim Naismith (University of St Andrews)

Excerpt: The new cell was a = 50.5 Å b = 65.1 Å c = 189.6 Å with space group P2₁2₁2₁. Old hands with burnt fingers will recognize that this looks very bad. The 90° image will be overlapped to an unusable extent as the long axis is parallel to the beam.The in-­‐house data collection set up came to the rescue. First we drove the χ axis (we have a partial χ goniometer) to its maximum deflection (45°) to try to move the long axis to a more amenable orientation. The image looked more hopeful but we knew we would still have to sacrifice resolution to ensure processability ( a painful compromise).

We were saved by another feature of our home source, the swing out angle. It allows higher resolution data to be collected with the detector positioned further back allowing better separation of spots. Such orientations of the detector are handled automatically in processing. By pushing the detector out 20° we obtained a new image. Although overlapping a little, the data were easily processed, we collected the data in three passes (different settings on the goniostat) to ensure completeness and redundancy. The new data gave spectacular quality maps and allowed us to tell the story.

Dr. Arwen Pearson from University of Leeds discusses her new HighFlux HomeLab system

Professor Bill Hunter
Biological Chemistry and Drug Discovery
College of Life Sciences, University of Dundee, Dundee

The decision to purchase from Rigaku was based on several criteria. Our group in Dundee requires equipment that would provide excellent in-house data collection including suitable automation, where high throughput is needed in support of early stage drug discovery. We need the capability to obtain highly redundant data for in-house anomalous dispersion phasing and the capacity to test and characterise large numbers of crystals from our more challenging problems in a manner that ensures we can optimise use of synchrotron time. The configuration from Rigaku matched perfectly to our multi-purpose requirements. My very positive experience of support from the company for almost 20 years now, also provided a degree of security in the decision.

Dean of Research in the College of Life Sciences, Prof. Mike Ferguson and
the Scientific Officer in Structural Biology, Dr Paul Fyfe with the newly commissioned
Rigaku X-ray generator, detector and sample changer.