Skip to main content

Catalase data collection & 1.7 Å structure

AppNote PX019 Using the EIGER 4M for single crystal X-ray diffraction on catalase

Background

Hybrid Photon Counting (HPC) detectors provide state-of-the-art technology for X-ray detection in the home X-ray crystallography laboratory. First, each pixel is a direct X-ray photon counter. Second, there is no readout noise or dark current which improves the signal-to-noise ratio in reflection measurements. Third, the single pixel point-spread function yields excellent spatial resolution of reflections. Fourth, the quick readout times allow for shutterless collection and shorter wall-times for diffraction experiments. The Dectris EIGER R 4M is currently the largest HPC detector with the smallest pixel size (75 x 75 μm²) available for the home X-ray laboratory. The large imaging size has the advantage of increasing the redundancy and resolution of reflections recorded in a single scan. The small pixel size has the advantage of allowing closer crystal to detector distances which decrease the number of images and time required to reach higher resolution data. To illustrate these advantages, we have collected data on a catalase crystal using the EIGER R 4M and solved its structure to 1.7 Å.

Protein crystallography products from Rigaku

A customized single crystal X-ray diffraction system that has the capabilities of utilizing both ports of a rotating anode X-ray source.

A microfocus sealed tube single crystal X-ray diffractometer that is low maintenance and perfect for screening and evaluating protein crystals.

A single crystal X-ray diffractometer with high-flux microfocus rotating anode X-ray generator and direct detection, HPC X-ray detector.

A versatile and high-flux dual-wavelength (DW) X-ray diffractometer with an HPC X-ray detector for multipurpose diffraction experiments

A leading provider of reagent kits, stock solutions, proteins, and accessories for protein researchers.

Small angle X-ray scattering (SAXS) Kratky camera system

User-inspired data collection and data processing software for small molecule and protein crystallography

Automated tool for performing in situ crystallography experiments on existing X-ray diffractometers

Robotic sample changer to provide unattended data acquisition, enhanced productivity and standardized workflow to your research environment.

A unique curved single crystal X-ray diffraction detector based on direct X-ray detection technology with the highest 2θ range at a single position available for the home lab.

Extremely low noise, air-cooled X-ray detector based on direct X-ray detection technology.

High-resolution X-ray detectors for X-ray diffractometers from DECTRIS

Automated crystal transport, orientation and retrieval robot

The smallest detachable motorized goniometer head on the market